Mouse TrkB ELISA Kit (ab203368)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 33.3 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse TrkB ELISA Kit
See all TrkB kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 4.4% Inter-assay Sample n Mean SD CV% Overall 3 3.2% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
33.3 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Mouse brain extract 106 103% - 110% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse TrkB ELISA Kit (ab203368) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of TrkB protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse TrkB with 33.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
TrkB is a tyrosine kinase receptor involved in the development of the peripheral and central nervous system. TrkB plays a role in synapse plasticity and formation, and neuron survival, proliferation and differentiation. Ligand (including BDNF and NTF4) interactions induces homodimerization, autophosphorylation and activation of TrkB. Activity of TrkB is also affected by the formation of inactive heterodimers with noncatalytic isoforms.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse TrkB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Mouse TrkB Capture Antibody (Lyophilized) 1 vial Mouse TrkB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1. -
Tissue specificity
Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsLigand-mediated auto-phosphorylation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- AI848316
- BDNF tropomyosine receptor kinase B
- BDNF/NT 3 growth factors receptor
see all -
Database links
- Entrez Gene: 18212 Mouse
- SwissProt: P15209 Mouse
- Unigene: 130054 Mouse
Images
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Other - Mouse TrkB ELISA Kit (ab203368)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse TrkB standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Specificity of mouse tissue samples within the working range of the assay.Interpolated values of TrkB are plotted based on an extract load of 50 µg/mL. Background-subtracted data values (mean +/- SD, n = 2) are graphed.
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Rat and mouse brain extract were diluted 2-fold in 1X Cell Extraction Buffer PTR and assayed for cross reactivity.Interpolated values from the mouse protein standard are graphed.
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Sandwich ELISA - Mouse TrkB ELISA Kit (ab203368)To learn more about the advantages of recombinant antibodies see here.
