Mouse NLRP3 ELISA Kit (ab279417)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.57 ng/ml
- Range: 3.91 ng/ml - 250 ng/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse NLRP3 ELISA Kit
See all NLRP3 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 4.7% Inter-assay Sample n Mean SD CV% Extract 3 4.9% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.57 ng/ml -
Range
3.91 ng/ml - 250 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 115 111% - 118% Cell Lysate 106 99% - 117% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse NLRP3 ELISA kit (ab279417) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Mouse NLRP3 protein in mouse cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse NLRP3 with 0.57 ng/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
NACHT, LRR and PYD domains-containing protein 3 (NLRP3), encoded by the NLRP3 gene, is known to have a vital role in the innate immunity and inflammation. NLRP3 is highly expressed in macrophages and as a component of the inflammasomes in which it functions to detect products of damaged cells and subsequently activates itself to trigger an immune response. Mutations in the NLRP3 has been shown in studies to be associated with a wide spectrum of dominantly inherited autoimmune diseases. The standard protein provided in ab279417 is the N-terminal 215 amino acids fragment of Mouse NLRP3.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse NLRP3 Capture Antibody 1 x 600µl 10X Mouse NLRP3 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Mouse NLRP3 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function as an inducer of apoptosis. Interacts selectively with ASC and this complex may function as an upstream activator of NF-kappa-B signaling. Inhibits TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. Also inhibits transcriptional activity of RELA. Activates caspase-1 in response to a number of triggers including bacterial or viral infection which leads to processing and release of IL1B and IL18. -
Tissue specificity
Expressed in blood leukocytes. Strongly expressed in polymorphonuclear cells and osteoblasts. Undetectable or expressed at a lower magnitude in B- and T-lymphoblasts, respectively. High level of expression detected in chondrocytes. Detected in non-keratinizing epithelia of oropharynx, esophagus and ectocervix and in the urothelial layer of the bladder. -
Involvement in disease
Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1) [MIM:120100]; also known as familial cold urticaria. FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold.
Defects in NLRP3 are a cause of Muckle-Wells syndrome (MWS) [MIM:191900]; also known as urticaria-deafness-amyloidosis syndrome. MWS is a hereditary periodic fever syndrome characterized by fever, chronic recurrent urticaria, arthralgias, progressive sensorineural deafness, and reactive renal amyloidosis. The disease may be severe if generalized amyloidosis occurs.
Defects in NLRP3 are the cause of chronic infantile neurologic cutaneous and articular syndrome (CINCA) [MIM:607115]; also known as neonatal onset multisystem inflammatory disease (NOMID). CINCA is a rare congenital inflammatory disorder characterized by a triad of neonatal onset of cutaneous symptoms, chronic meningitis and joint manifestations with recurrent fever and inflammation. -
Sequence similarities
Belongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- AGTAVPRL
- AII/AVP
- Angiotensin/vasopressin receptor AII/AVP like
see all -
Database links
- Entrez Gene: 216799 Mouse
- SwissProt: Q8R4B8 Mouse
- Unigene: 54174 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefits -
Example of mouse NLRP3 standard curve in 1X Cell Extraction Buffer.The NLRP3 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native NLRP3 in mouse J774A.1 stimulated and J774A.1 unstimulated cell extract based on a 500 µg/mL extract load.The concentrations of NLRP3 were measured in duplicate and interpolated from the NLRP3 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NLRP3 concentration was determined to be 187.6 ng/mL in J774A.1 stimulated cell extract and 5.45 ng/mL in J774A.1 unstimulated cell extract.