Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse NFAT2 Antibody Pair - BSA and Azide free (NFATC1) (ab253478)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

Overview

  • Product name

    Mouse NFAT2 Antibody Pair - BSA and Azide free (NFATC1)
    See all NFAT2 kits

  • Assay type

    ELISA set

  • Range

    54.688 pg/ml - 3500 pg/ml

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    The Antibody Pair can be used to quantify Mouse NFAT2. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277705), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse NFAT2 (NFATC1) Capture Antibody (unconjugated) 1 x 100µg
    Mouse NFAT2 (NFATC1) Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.

  • Tissue specificity

    Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

  • Sequence similarities

    Contains 1 RHD (Rel-like) domain.

  • Domain

    Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
    The N-terminal transactivation domain (TAD-A) binds to and is activated by Cbp/p300. The dephosphorylated form contains two unmasked nuclear localization signals (NLS), which allow translocation of the protein to the nucleus.
    Isoforms C have a C-terminal part with an additional trans-activation domain, TAD-B, which acts as a transcriptional activator. Isoforms B have a shorter C-terminal part without complete TAD-B which acts as a transcriptional repressor.

  • Post-translational
    modifications

    Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
  • Information by UniProt

  • Alternative names

    • cytoplasmic 1
    • MGC138448
    • NF ATc
    • NF ATc1
    • NF-ATc
    • NF-ATc1
    • NF-ATc1.2
    • NFAC1_HUMAN
    • NFAT 2
    • NFAT transcription complex cytosolic component
    • NFATc
    • NFATC 1
    • NFATc1
    • Nuclear factor of activated T cells cytoplasmic 1
    • Nuclear factor of activated T cells cytoplasmic calcineurin dependent 1
    • Nuclear factor of activated T cells cytosolic component 1
    • Nuclear factor of activated T-cells
    • nuclear factor of activated T-cells 'c'
    see all

  • Database links

    Images

    • Example of Mouse NFAT2 (NFATC1) standard curve.
      Example of Mouse NFAT2 (NFATC1) standard curve.

      Representative standard curve from corresponding SimpleStep ELISA® Kit (ab277705), which uses the same antibody pair. Note that the reference range provided for the pair is an estimation based on the performance of the related kit.

    • Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253478)
      Sandwich ELISA - Antibody Pair - BSA and Azide Free (ab253478)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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