Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free (ab221848)
Overview
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Product name
Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control - BSA and Azide free
See all Coiled-coil domain-containing protein 113 isotype controls -
Specificity
This Mouse IgG1 Isotype Control Antibody was raised in mouse against a yeast-specific protein making it unsuitable for yeast experiments. -
Tested applications
Suitable for: IHC-P, ICC, Flow Cytmore details -
Immunogen
Other Immunogen Type. ab221848 binds specifically to DNA-binding domain of the yeast GAL4 protein transcription activator.
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General notes
This antibody clone is manufactured by Abcam.
ab221848 is a PBS only buffer formulated version of ab170190.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
15-6E10A7 -
Isotype
IgG1 -
Light chain type
kappa -
Alternative names
- CCDC113
- Coiled-coil domain-containing protein 113 Gene names
- DKFZp434N1418
- HSPC065
Images
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This flow cytometry data was generated using the same antibody clone under a different buffer format (cat# ab170190).
Flow cytometry experiments with 4% PFA fixed HeLa (Human adenocarcinoma), 653s (mouse myeloma), H4IIE (rat hepatoma) and H9C2 (rat myoblast) were performed with the IgG1 Isotype Control Antibody (red) and no primary antibody negative control (black).
An Alexa Fluor® 488 conjugate with isotype specificity to the mouse antibody was used as a secondary antibody. The isotype control at 1 ug/mL shows no higher signal than the no primary negative control.