Mouse IGFBP7 ELISA Kit (ab245712)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6.03 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse IGFBP7 ELISA Kit
See all IGFBP7 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 2.6% Inter-assay Sample n Mean SD CV% serum 3 11.2% -
Sample type
Cell culture supernatant, Urine, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6.03 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 83% - 100% Urine 102 100% - 104% Serum 106 103% - 111% Tissue Extracts 99 97% - 100% EDTA Plasma 97 92% - 104% Cit plasma 104 100% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow -
Product overview
Mouse IGFBP7 ELISA Kit (ab245712) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP7 protein in cell culture supernatant, cit plasma, edta plasma, serum, tissue extracts, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP7 with 6.03 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse IGFBP7 Capture Antibody 1 x 600µl 10X Mouse IGFBP7 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse IGFBP7 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- AGM
- Angiomodulin
- FSTL2
see all -
Database links
- Entrez Gene: 29817 Mouse
- SwissProt: Q61581 Mouse
- Unigene: 233470 Mouse
Images
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Other - Mouse IGFBP7 ELISA Kit (ab245712)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse IGFBP7 standard curve in Sample Diluent NS.The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of mouse IGFBP7 standard curve in 1X Cell Extraction Buffer PTR.The IGFBP7 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native IGFBP7 in mouse serum, plasma, urine, and cell culture supernatant samples.The concentrations of IGFBP7 were measured in duplicates, interpolated from the IGFBP7 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, urine 1%, and 3T3-L1 cell culture supernatant 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 87.42 ng/mL in serum, 74.99 ng/mL in plasma (citrate), 85.41 ng/mL in plasma (EDTA) 28.69 ng/mL in urine, and 24.46 ng/mL in 3T3-L1 cell culture supernatant. -
Interpolated concentrations of native IGFBP7 in mouse lung extract (25 ug/mL load) and spleen extract (50 ug/mL load).The concentrations of IGFBP7 were measured in duplicate and interpolated from the IGFBP7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP7 concentration was determined to be 2039.98 pg/mL in lung extract and 2422.48 pg/mL in spleen extract. -
Sandwich ELISA - Mouse IGFBP7 ELISA Kit (ab245712)To learn more about the advantages of recombinant antibodies see here.
