Anti-MafA antibody (ab26405)
Key features and details
- Rabbit polyclonal to MafA
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MafA antibody
See all MafA primary antibodies -
Description
Rabbit polyclonal to MafA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human MafA (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab28523)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa). Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). The mouse protein has also been observed at 47 kDa according to reference PMID 12368292.
IHC-P Use a concentration of 0.8 - 4 µg/ml. Target
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Function
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. -
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsUbiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. -
Cellular localization
Nucleus. Detected in nuclei of pancreas islet beta cells. - Information by UniProt
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Database links
- Entrez Gene: 389692 Human
- Entrez Gene: 378435 Mouse
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- SwissProt: Q8CF90 Mouse
- Unigene: 521914 Human
- Unigene: 309589 Mouse
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Alternative names
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (ab26405)The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
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Western blot - Anti-MafA antibody (ab26405)Anti-MafA antibody (ab26405) at 1 µg/ml + Brain (Human) Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID:14680841, PMID:18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
Protocols
References (11)
ab26405 has been referenced in 11 publications.
- Lin JY et al. In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals. Cell Discov 6:20 (2020). PubMed: 32284878
- Dirice E et al. Human duct cells contribute to ß cell compensation in insulin resistance. JCI Insight 4:N/A (2019). PubMed: 30996131
- Axelsson AS et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun 8:15652 (2017). WB, IHC-P . PubMed: 28585545
- Hao T et al. Glucagon-like peptide 1 receptor agonist ameliorates the insulin resistance function of islet ß cells via the activation of PDX-1/JAK signaling transduction in C57/BL6 mice with high-fat diet-induced diabetes. Int J Mol Med 39:1029-1036 (2017). PubMed: 28290604
- Cinti F et al. Evidence of ß-Cell Dedifferentiation in Human Type 2 Diabetes. J Clin Endocrinol Metab 101:1044-54 (2016). PubMed: 26713822
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody (ab26405)The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
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Western blot - Anti-MafA antibody (ab26405)Anti-MafA antibody (ab26405) at 1 µg/ml + Brain (Human) Tissue Lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID:14680841, PMID:18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
