Anti-MafA antibody [BLR067G] - BSA free (ab264418)
![Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-366130-AntiMafA-antibody-BLR067G-western-blot-betatc6-mouse.jpg "Anti-MafA antibody [BLR067G] - BSA free (ab264418)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR067G] to MafA - BSA free
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MafA antibody [BLR067G] - BSA free
See all MafA primary antibodies -
Description
Rabbit monoclonal [BLR067G] to MafA - BSA free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P MouseHumanWB MouseHuman
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Immunogen
Synthetic peptide within Human MafA aa 125-175. The exact sequence is proprietary. NP_963883.2.
Database link: Q8NHW3 -
Positive control
- IHC-P: Human and mouse pancreas tissue; Human pancreatic tumor tissue; Human pancreatic carcinoma tissue. ICC: Mouse beta-TC-6 cells. WB: Mouse beta-TC-6 whole cell lysate; HEK-293T whole cell lysate.
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General notes
This product is sold under License from Bethyl Laboratories, Inc
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR067G -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)All lanes : Anti-MafA antibody [BLR067G] - BSA free (ab264418) at 1/1000 dilution
Lane 1 : Beta-TC-6 whole cell lysate at 10 µg
Lane 2 : AlphaTC1 clone 9 whole cell lysate at 50 µg
Lane 3 : NIH/3T3 whole cell lysate at 50 µg
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-4-anti-mafa-antibody-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic carcinoma tissue labeling MafA with ab264418 at 1/250 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-3-anti-mafa-antibody-immunohistochemistry-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)Immunohistochemical analysis of JB zinc-fixed, paraffin-embedded mouse pancreas tissue labeling MafA with ab264418 at 1/200 dilution. Detection: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-2-anti-mafa-antibody-immunohistochemistry-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreatic tumor tissue labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-5-anti-mafa-antibody-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-MafA antibody [BLR067G] - BSA free (ab264418)Immunocytochemical analysis of formalin-fixed, paraffin-embedded mouse beta-TC-6 cells labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-1-anti-mafa-antibody-immunohistochemistry-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MafA antibody [BLR067G] - BSA free (ab264418)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human pancreas tissue labeling MafA with ab264418 at 1/200 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
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![Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-366131-AntiMafA-antibody-BLR067G-western-blot-transfected.jpg)
Western blot - Anti-MafA antibody [BLR067G] - BSA free (ab264418)All lanes : Anti-MafA antibody [BLR067G] - BSA free (ab264418) at 1/1000 dilution
Lane 1 : Whole cell lysate from HEK-293T transfected with human MafA
Lane 2 : Whole cell lysate from HEK-293T transfected with human MafB
Lane 3 : Whole cell lysate from HEK-293T transfected with human MafC
Lane 4 : Whole cell lysate from HEK-293T transfected with mouse MafA
Lane 5 : Whole cell lysate from HEK-293T transfected with mouse MafB
Lane 6 : Whole cell lysate from HEK-293T transfected with mouse MafC
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 secondsLysates prepared using NETN lysis buffer.
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![Anti-MafA antibody [BLR067G] - BSA free (ab264418)](https://www.abcam.com/ps/products/264/ab264418/Images/ab264418-8-benefits-of-recombinant-antibodies.png)
Anti-MafA antibody [BLR067G] - BSA free (ab264418)
