Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUDT9 antibody [EPR15175] (ab197021)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15175] to NUDT9
  • Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-NUDT9 antibody [EPR15175]
    See all NUDT9 primary antibodies

  • Description

    Rabbit monoclonal [EPR15175] to NUDT9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat and HUVEC cell lysates. IHC-P: Human and rat kidney tissues. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NUDT9 antibody [EPR15175] (ab197021)
    Western blot - Anti-NUDT9 antibody [EPR15175] (ab197021)
    All lanes : Anti-NUDT9 antibody [EPR15175] (ab197021) at 1/20000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUDT9 antibody [EPR15175] (ab197021)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling NUDT9 with ab197021 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Rat kidney tissue is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUDT9 antibody [EPR15175] (ab197021)
    Immunocytochemistry/ Immunofluorescence - Anti-NUDT9 antibody [EPR15175] (ab197021)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUDT9 with ab197021 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).  Mitochondrion staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). COX IV is detected with ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab197021 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab33985 (anti-COX IV mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-NUDT9 antibody [EPR15175] (ab197021)
    Flow Cytometry - Anti-NUDT9 antibody [EPR15175] (ab197021)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUDT9 with ab197021 at 1/170 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Anti-NUDT9 antibody [EPR15175] (ab197021)
    Anti-NUDT9 antibody [EPR15175] (ab197021)

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