Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MPZL antibody [EPR9229] (ab151541)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9229] to MPZL
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MPZL antibody [EPR9229]
    See all MPZL primary antibodies

  • Description

    Rabbit monoclonal [EPR9229] to MPZL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human MPZL aa 200-300 (intracellular). The exact sequence is proprietary.

  • Positive control

    • WB: 293T, HeLa and HepG2 whole cell lysate (ab7900). ICC/IF: HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR9229

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MPZL antibody [EPR9229] (ab151541)
    Western blot - Anti-MPZL antibody [EPR9229] (ab151541)
    All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MPZL1 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab151541 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa. 

     ab151541 Anti-MPZL antibody [EPR9229] was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265332 (knockout cell lysate ab258053) was used.  Wild-type and MPZL knockout samples were subjected to SDS-PAGE.  ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-MPZL antibody [EPR9229] (ab151541)
    Immunocytochemistry/ Immunofluorescence - Anti-MPZL antibody [EPR9229] (ab151541)
    Immunofluorescent analysis of HepG2 cells labeling MPZL with ab151541 at 1/250 dilution.
  • Western blot - Anti-MPZL antibody [EPR9229] (ab151541)
    Western blot - Anti-MPZL antibody [EPR9229] (ab151541)
    All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 29 kDa

  • Anti-MPZL antibody [EPR9229] (ab151541)
    Anti-MPZL antibody [EPR9229] (ab151541)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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