Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9229] to MPZL
Suitable for: WB, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-MPZL antibody [EPR9229]
See all MPZL primary antibodies
Description

Rabbit monoclonal [EPR9229] to MPZL
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human MPZL aa 200-300 (intracellular). The exact sequence is proprietary.
Positive control
- WB: 293T, HeLa and HepG2 whole cell lysate (ab7900). ICC/IF: HepG2 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR9229
Isotype

IgG
Research areas

Western blot - Anti-MPZL antibody [EPR9229] (ab151541)

All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : MPZL1 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 29 kDa
Observed band size: 37 kDa
why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab151541 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.

ab151541 Anti-MPZL antibody [EPR9229] was shown to specifically react with MPZL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265332 (knockout cell lysate ab258053) was used. Wild-type and MPZL knockout samples were subjected to SDS-PAGE. ab151541 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-MPZL antibody [EPR9229] (ab151541)

Immunofluorescent analysis of HepG2 cells labeling MPZL with ab151541 at 1/250 dilution.
Western blot - Anti-MPZL antibody [EPR9229] (ab151541)

All lanes : Anti-MPZL antibody [EPR9229] (ab151541) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 29 kDa
Anti-MPZL antibody [EPR9229] (ab151541)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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