Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse IGFBP5 ELISA Kit (ab208345)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 73 pg/ml
  • Range: 125 pg/ml - 8000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse IGFBP5 ELISA Kit
    See all IGFBP5 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Mouse serum 5 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Mouse serum 3 2.4%

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    73 pg/ml

  • Range

    125 pg/ml - 8000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 104 102% - 106%
    Serum 88 83% - 96%
    Hep Plasma 93 80% - 104%
    Cit plasma 98 92% - 102%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse
    Does not react with: Goat, Cow, Pig

  • Product overview

    Mouse IGFBP5 ELISA Kit (ab208345) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IGFBP5 protein in cell culture supernatant, serum, and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse IGFBP5 with 73 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Insulin-like growth factor-binding protein 5 (IGFBP5) is an IGF binding protein involved in prolonging its half-life, and has both inhibitory and stimulatory effects on IGFs. IGFBP5 can also alter the interaction of IGFs with their receptors.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Mouse IGFBP5 ELISA Kit (ab208345)
    Other - Mouse IGFBP5 ELISA Kit (ab208345)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of mouse IGFBP5 standard curve
    Example of mouse IGFBP5 standard curve

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native IGFBP5 in mouse serum and plasma samples
    Interpolated concentrations of native IGFBP5 in mouse serum and plasma samples

    The concentrations of IGFBP5 were measured in duplicates, interpolated from the IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:600, plasma 1:800, and plasma (heparin) 1:800. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2,058 ng/mL in serum, 3,670 ng/mL in plasma (citrate), and 3,865 ng/mL in plasma (heparin).

  • This assay detects rat IGFBP5
    This assay detects rat IGFBP5

    The concentrations of IGFBP5 in rat serum were measured in duplicates, interpolated from the mouse IGFBP5 standard curves and corrected for sample dilution. Undiluted samples are rat serum 1:250. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IGFBP5 concentration was determined to be 2691 ng/mL in rat serum.

  • Sandwich ELISA - Mouse IGFBP5 ELISA Kit (ab208345)
    Sandwich ELISA - Mouse IGFBP5 ELISA Kit (ab208345)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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