Mouse IGFBP5 Matched Antibody Pair Kit (ab215411)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Mouse IGFBP5 Matched Antibody Pair Kit
See all IGFBP5 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
41.6 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse IGFBP5 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse IGFBP5.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab208345. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse IGFBP5 Capture Antibody 2 x 50µg 1 x 50µg Mouse IGFBP5 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse IGFBP5 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Tissue specificity
Osteosarcoma, and at lower levels in liver, kidney and brain. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IBP 5
- IBP-5
- IBP5
see all -
Database links
- Entrez Gene: 16011 Mouse
- SwissProt: Q07079 Mouse
- Unigene: 405761 Mouse
Images
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Mouse IGFBP5 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse IGFBP5 Matched Antibody Pair Kit (ab215411)To learn more about the advantages of recombinant antibodies see here.
