Mouse Fas Ligand Antibody Pair - BSA and Azide free (FasL) (ab253326)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse
Overview
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Product name
Mouse Fas Ligand Antibody Pair - BSA and Azide free (FasL)
See all Fas Ligand kits -
Assay type
ELISA set -
Range
18.75 pg/ml - 1200 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
The Antibody Pair can be used to quantify Mouse Fas Ligand (FasL). BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab270894), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Mouse Fas Ligand Capture Antibody (unconjugated) 1 x 100µg Mouse Fas Ligand Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
Involvement in disease
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsN-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
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Alternative names
- ALPS1B
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
see all -
Database links
- Entrez Gene: 14103 Mouse
- SwissProt: P41047 Mouse
- Unigene: 3355 Mouse
Images
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Sandwich ELISA - Mouse Fas Ligand Antibody Pair - BSA and Azide free (FasL) (ab253326)To learn more about the advantages of recombinant antibodies see here.
