Mouse Fas Ligand ELISA Kit (FasL) (ab270894)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.7 pg/ml
- Range: 18.75 pg/ml - 1200 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Fas Ligand ELISA Kit (FasL)
See all Fas Ligand kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.9% Inter-assay Sample n Mean SD CV% Serum 3 5.4% -
Sample type
Cell culture supernatant, Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.7 pg/ml -
Range
18.75 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105 98% - 121% Cell culture media 101 96% - 104% Hep Plasma 101 99% - 104% EDTA Plasma 90 88% - 93% Cit plasma 99 96% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Fas Ligand ELISA kit (ab270894) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fas Ligand protein in mouse serum, plasma and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate mouse Fas Ligand with 5.7 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Fas Ligand (FasL), also known as Tumor necrosis factor ligand superfamily member 6 or CD95 ligand is a cytokine that binds to TNFRSF6/FAS/CD95, a receptor that transduces the apoptotic signal into cells. Fas Ligand is involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development. It also initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses. TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance. Rat Fas Ligand has 93% amino acids sequence identity with mouse Fas Ligand.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Fas Ligand (FasL) Capture Antibody 1 x 600µl 10X Mouse Fas Ligand (FasL) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse Fas Ligand (FasL) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. -
Involvement in disease
Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsN-glycosylated.
The soluble form derives from the membrane form by proteolytic processing. -
Cellular localization
Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface. - Information by UniProt
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Alternative names
- ALPS1B
- Apoptosis (APO 1) antigen ligand 1
- Apoptosis antigen ligand
see all -
Database links
- Entrez Gene: 14103 Mouse
- SwissProt: P41047 Mouse
- Unigene: 3355 Mouse
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse Fas ligand competitor curve comparisonStandard Curve comparison between Mouse Fas Ligand SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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. Example of mouse Fas Ligand standard curve in Sample Diluent NS.The Fas Ligand standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Fas Ligand in mouse spleen tissue culture supernatant samples treated with or without PMA (50 ng/mL) and calcium ionomycin (500 ng/mL) for 3 days.The concentrations of Fas Ligand were measured in duplicates, interpolated from the Fas Ligand standard curves and corrected for sample dilution. Undiluted samples are as follows: treated and untreated spleen supernatants 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fas Ligand concentration was determined to be 38 pg/mL in treated spleen supernatant and undetectable in untreated spleen supernatant.
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Sandwich ELISA - Mouse Fas Ligand ELISA Kit (FasL) (ab270894)To learn more about the advantages of recombinant antibodies see here.
