JNK2 ELISA Kit (ab218265)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 80.7 pg/ml
- Range: 234 pg/ml - 15000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
JNK2 ELISA Kit
See all JNK2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% MCF7 extract 5 2.4% Inter-assay Sample n Mean SD CV% MCF7 extract 3 11.9% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
80.7 pg/ml -
Range
234 pg/ml - 15000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 96 93% - 110% Tissue Extracts 103 100% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
JNK2 ELISA Kit (ab218265) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of JNK2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate JNK2 with 80.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
JNK2 is a c-Jun N-terminal kinase related to JNK1 and JNK3; all are mitogen-activated protein kinase family members. The JNKs are activated in response to stress stimuli, including inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock. JNK2 functions in signal transduction be phosphorylating transcription factors. JNK2 amino acid sequence is highly conserved across mammals and human JNK2 has approximately 81% sequence identity to human JNK1 and 84% to JNK3.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X JNK2 Capture Antibody 1 x 600µl 10X JNK2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml JNK2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. - Information by UniProt
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Alternative names
- c Jun kinase 2
- C Jun N terminal kinase 2
- c-Jun N-terminal kinase 2
see all -
Database links
- Entrez Gene: 5601 Human
- Entrez Gene: 26420 Mouse
- Entrez Gene: 50658 Rat
- Omim: 602896 Human
- SwissProt: P45984 Human
- SwissProt: Q9WTU6 Mouse
- SwissProt: P49186 Rat
- Unigene: 484371 Human
see all
Images
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Other - JNK2 ELISA Kit (ab218265)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of JNK2 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native JNK2 in human brain homogenate extract, MCF7 extract, and HEPG2 extract.Undiluted extract based on an 800 μg/mL, 300 μg/mL, and 800 μg/mL respective extract load. The concentrations of JNK2 were measured in duplicate and interpolated from the JNK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JNK2 concentration was determined to be 5175 pg/mL in human brain homogenate extract, 10721 pg/mL in MCF7 extract and 1451 pg/mL in HEPG2 extract.
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Interpolated concentrations of native JNK2 in mouse NIH/3T3 cell extract and rat PC-12 cell extract based on a 400 µg/mL extract load.The concentrations of JNK2 were measured in duplicate and interpolated from the JNK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JNK2 concentration was determined to be 7288 pg/mL in mouse NIH/3T3.
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Sandwich ELISA - JNK2 ELISA Kit (ab218265)To learn more about the advantages of recombinant antibodies see here.
