Astana Biomed Group, an authorized Abcam distributor in Central Asia

JNK2 ELISA Kit (ab218265)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 80.7 pg/ml
  • Range: 234 pg/ml - 15000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    JNK2 ELISA Kit
    See all JNK2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    MCF7 extract 5 2.4%
    Inter-assay
    Sample n Mean SD CV%
    MCF7 extract 3 11.9%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    80.7 pg/ml

  • Range

    234 pg/ml - 15000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 96 93% - 110%
    Tissue Extracts 103 100% - 105%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Product overview

    JNK2 ELISA Kit (ab218265) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of JNK2 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate JNK2 with 80.7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    JNK2 is a c-Jun N-terminal kinase related to JNK1 and JNK3; all are mitogen-activated protein kinase family members.  The JNKs are activated in response to stress stimuli, including inflammatory cytokines, ultraviolet irradiation, heat shock, and osmotic shock.  JNK2 functions in signal transduction be phosphorylating transcription factors.  JNK2 amino acid sequence is highly conserved across mammals and human JNK2 has approximately 81% sequence identity to human JNK1 and 84% to JNK3.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - JNK2 ELISA Kit (ab218265)
    Other - JNK2 ELISA Kit (ab218265)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of JNK2 standard curve.
    Example of JNK2 standard curve.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native JNK2 in human brain homogenate extract, MCF7 extract, and HEPG2 extract.
    Interpolated concentrations of native JNK2 in human brain homogenate extract, MCF7 extract, and HEPG2 extract.

    Undiluted extract based on an 800 μg/mL, 300 μg/mL, and 800 μg/mL respective extract load. The concentrations of JNK2 were measured in duplicate and interpolated from the JNK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JNK2 concentration was determined to be 5175 pg/mL in human brain homogenate extract, 10721 pg/mL in MCF7 extract and 1451 pg/mL in HEPG2 extract.

  • Interpolated concentrations of native JNK2 in mouse NIH/3T3 cell extract and rat PC-12 cell extract based on a 400 µg/mL extract load.
    Interpolated concentrations of native JNK2 in mouse NIH/3T3 cell extract and rat PC-12 cell extract based on a 400 µg/mL extract load.

    The concentrations of JNK2 were measured in duplicate and interpolated from the JNK2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean JNK2 concentration was determined to be 7288 pg/mL in mouse NIH/3T3.

  • Sandwich ELISA - JNK2 ELISA Kit (ab218265)
    Sandwich ELISA - JNK2 ELISA Kit (ab218265)
    To learn more about the advantages of recombinant antibodies see here.

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