JNK2 Matched Antibody Pair Kit (ab220134)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse, Rat, Human
- Range: 125 ng/ml - 8000 ng/ml
Overview
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Product name
Human JNK2 Matched Antibody Pair Kit
See all JNK2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.47 pg/ml -
Range
125 ng/ml - 8000 ng/ml -
Species reactivity
Reacts with: Human -
Product overview
Human JNK2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human JNK2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab218265. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human JNK2 Capture Antibody 2 x 50µg 1 x 50µg Human JNK2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human JNK2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. - Information by UniProt
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Alternative names
- c Jun kinase 2
- C Jun N terminal kinase 2
- c-Jun N-terminal kinase 2
see all -
Database links
- Entrez Gene: 5601 Human
- Omim: 602896 Human
- SwissProt: P45984 Human
- Unigene: 484371 Human
Images
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Human JNK2 standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human JNK2 Matched Antibody Pair Kit (ab220134)To learn more about the advantages of recombinant antibodies see here.
