Anti-CAST antibody (ab236662)
Key features and details
- Rabbit polyclonal to CAST
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-CAST antibody
See all CAST primary antibodies -
Description
Rabbit polyclonal to CAST -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P Human
-
Immunogen
Recombinant fragment corresponding to Human CAST aa 250-450.
Database link: O15446 -
Positive control
- IHC-P: Human breast cancer tissue. ICC/IF: HeLa cells.
-
General notes
Previously labelled as PAF49.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (ab236662)
Paraffin-embedded human breast cancer tissue stained for CAST using ab236662 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
-
Immunocytochemistry/ Immunofluorescence - Anti-CAST antibody (ab236662)HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for CAST using ab236662 at a dilution of 1/66 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
