Anti-CAST antibody (ab76933)
Key features and details
- Rabbit polyclonal to CAST
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CAST antibody
See all CAST primary antibodies -
Description
Rabbit polyclonal to CAST -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide corresponding to Human CAST. corresponding to a region between residues 404 and 454
Database link: NP_036231.1 -
Positive control
- HeLa whole cell lysate.
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General notes
Previously labelled as PAF49.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab76933 was affinity purified using an epitope specific to CAST immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAST antibody (ab76933)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling CAST with ab76933 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CAST antibody (ab76933)All lanes : Anti-CAST antibody (ab76933) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 55 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-CAST antibody (ab76933)ab76933 at 1µg/ml staining CAST in HeLa whole cell lysate immunoprecipitated using ab76933 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
