Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23811-15] to SIRT7 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free
    See all SIRT7 primary antibodies

  • Description

    Rabbit monoclonal [EPR23811-15] to SIRT7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hepa1-6, Neuro-2a, NIH/3T3, RAW 264.7, C6, HeLa, HEK-293T, HepG2 and MCF7 whole cell lysates; Mouse spleen, skeletal muscle and liver tissue lysates; Ratskeletal muscle and liver tissue lysates. IHC-P: Mouse liver tissue; Rat spleen tissue. IP: HeLa and Neuro-2a whole cell lysates.

  • General notes

    ab275035 is the carrier-free version of ab259968.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in mouse liver (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    SIRT7 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate 10 ug

    Lane 2: ab259968 IP in Neuro-2a whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 67 seconds.

     

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-CD16+CD32 antibody [93] (ab269968) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat liver tissue lysate
    Lane 5 : Rat skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    Low expression: skeletal muscle (PMID: 16618798).

  • Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Western blot - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    All lanes : Anti-SIRT7 antibody [EPR23811-15] (ab259968) at 1/1000 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
    Lane 3 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
    Lane 4 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 5 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
    Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression pattern of SIRT7 is consistent with what has been described in the literature(PMID: 24771643, 16618798).

  • Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunoprecipitation - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    SIRT7 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate with ab259968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259968 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab259968 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259968 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

    This data was developed using ab259968, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling SIRT7 with ab259968 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining in rat spleen (PMID: 16618798). The section was incubated with ab259968 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)
    Anti-SIRT7 antibody [EPR23811-15] - BSA and Azide free (ab275035)

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