Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7308(2)] to Frizzled 8 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free
    See all Frizzled 8 primary antibodies

  • Description

    Rabbit monoclonal [EPR7308(2)] to Frizzled 8 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species. 

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human normal pancreas tissue and Human breast carcinoma.

  • General notes

    Ab238967 is the carrier-free version of ab155093. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238967 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Frizzled 8 with Purified ab155093 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

    Immunohistochemical analysis of paraffin embedded human normal pancreas tissue using ab155093 at a 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, tissue culture supernatant and sodium azide (ab155093).

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)
    Anti-Frizzled 8 antibody [EPR7308(2)] - BSA and Azide free (ab238967)

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