Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7013] to Endothelin B Receptor/ET-B - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free
    See all Endothelin B Receptor/ET-B primary antibodies

  • Description

    Rabbit monoclonal [EPR7013] to Endothelin B Receptor/ET-B - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248300 is the carrier-free version of ab129102 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248300 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.21 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR7013

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/10000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    This data was developed using ab129102, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    Immunoprecipitation - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    This data was developed using ab129102, the same antibody clone in a different buffer formulation.ab129102 (purified) at 1/40 immunoprecipitating Endothelin B receptor//ET-B in 10 μg 293T (Lanes 1 and 2, observed at 50 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    Western blot - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    All lanes : Anti-Endothelin B Receptor/ET-B antibody [EPR7013] (ab129102) at 1/1000 dilution (unpurified)

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : JAR cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab129102, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    OI-RD Scanning - Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    This data was developed using ab129102, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)
    Anti-Endothelin B Receptor/ET-B antibody [EPR7013] - BSA and Azide free (ab248300)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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