Anti-Frizzled 8 antibody (ab40012)
Key features and details
- Goat polyclonal to Frizzled 8
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Frizzled 8 antibody
See all Frizzled 8 primary antibodies -
Description
Goat polyclonal to Frizzled 8 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide:
SYPKQMPLSQV
, corresponding to C terminal amino acids 684-694 of Human Frizzled 8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Frizzled 8 antibody (ab40012)Anti-Frizzled 8 antibody (ab40012) at 1 µg/ml + Human Pancreas lysate in RIPA buffer at 35 µg
Predicted band size: 73 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody (ab40012)ab40012 at 3µg/ml staining Frizzled 8 in human pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
