Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7308(2)] to Frizzled 8
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Frizzled 8 antibody [EPR7308(2)]
    See all Frizzled 8 primary antibodies

  • Description

    Rabbit monoclonal [EPR7308(2)] to Frizzled 8

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the 100% homology with the immunogen. We do not have experimental data in mouse and rat species.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Frizzled 8 aa 650-750. The exact sequence is proprietary.

  • Epitope

    HeLa and Jurkat lysates; Human endometrial carcinoma tissue.

  • Positive control

    • WB: Jurkat whole cell lysate (ab7899), HeLa, 293T, PC-3, Human breast cancer lysates, Human prostate cancer lysates, Human prostate lysates, Human lung cancer lysates, Human lung lysates IHC-P: Human breast carcinoma

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovations Ltd. Rhodadyn™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Frizzled 8 with Purified ab155093 at 1:500 dilution (1.17 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

    Lane 1 : Human prostate cancer lysates
    Lane 2 : Human prostate lysates
    Lane 3 : Human lung cancer lysates
    Lane 4 : Human lung lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa



    The expression level of Frizzled 8 in normal tissues is low and can be upregulated in cancer tissues reported by PMID 23815780 and 22138402

  • Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 3 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
    Lane 4 : Human breast cancer lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 73 kDa
    Observed band size: 73 kDa

  • Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Western blot - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    All lanes : Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 73 kDa



    Primary antibody diluted with 1% BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling Frizzled 8 with ab155093 at 1/50 dilution.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue using ab155093 showing +ve staining.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab155093 showing +ve staining.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155093 showing +ve staining.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155093 showing +ve staining.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

    Immunohistochemical analysis of paraffin embedded human normal pancreas tissue using ab155093 showing +ve staining.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)
    Anti-Frizzled 8 antibody [EPR7308(2)] (ab155093)

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