Human YWHAG (14-3-3 gamma) knockout HEK-293T cell lysate (ab257805)
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg ab260354 - Human YWHAG knockout HEK293T cell lysate 1 x 100µg -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
-
Western blot - Human YWHAG (14-3-3 gamma) knockout HEK293T cell lysate (ab257805)
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:YWHAG knockout HEK293T cell lysate (20 ug)
Lane 3:A431 cell lysate (20 ug)
Lane 4:HeLa cell lysate (20 ug)ab137048 was shown to specifically react with 14-3-3 gamma/YWHAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266734 (knockout cell lysate ab257805) was used. Wild-type and 14-3-3 gamma/YWHAG knockout samples were subjected to SDS-PAGE. ab137048 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Sanger Sequencing - Human YWHAG knockout HEK293T cell lysate (ab257805)Homozygous: 1 bp insertion in exon1
