Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
![Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-410489-anti-spry-2-antibody-epr4318-2-b-bsa-and-azide-free-western-blot.jpg "Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4318(2)(B)] to Spry-2 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Rat, Human
Overview
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Product name
Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free
See all Spry-2 primary antibodies -
Description
Rabbit monoclonal [EPR4318(2)(B)] to Spry-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250224 is the carrier-free version of ab180527. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250224 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4318(2)(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
This data was developed using ab180527, the same antibody clone in a different buffer formulation.
Western blot analysis of HeLa cell lysate immunoprecipitated with ab180527. Detection utilised ab180527 at 1/40 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-4-anti-spry-2-antibody-epr4318-2-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)This data was developed using ab180527, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of paraformaldehyde-fixed SH-SY5Y cells labeling Spry-2 with ab180527 at 1/1000 dilution. Goat anti-rabbit IgG (Dylight 488) at 1/250 dilution was used as the secondary antibody. The slide is counterstained with Dapi. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-3-anti-spry-2-antibody-epr4318-2-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)This data was developed using ab180527, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Spry-2 with ab180527 at 1/50 dilution. The retrieval buffer was 0.01 M Tris-EDTA (pH 9.0) and HIER was performed in a Decloaker (125°C, 30 sec, cool down to room temperature for 10-30 min). The slide is counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-402557-anti-spry-2-antibody-epr4318-2-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDaThis data was developed using ab180527, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-398376-anti-spry-2-antibody-epr4318-2-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)All lanes : Anti-Spry-2 antibody [EPR4318(2)(B)] (ab180527) at 1/2000 dilution
Lane 1 : HT-29 cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 35 kDaThis data was developed using ab180527, the same antibody clone in a different buffer formulation.
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![Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)](https://www.abcam.com/ps/products/250/ab250224/Images/ab250224-6-benefits-of-recombinant-antibodies.png)
Anti-Spry-2 antibody [EPR4318(2)(B)] - BSA and Azide free (ab250224)
