Human sTNF RII (TNFRSF1B) Antibody Pair - BSA and Azide free (ab267685)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
Overview
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Product name
Human sTNF RII (TNFRSF1B) Antibody Pair - BSA and Azide free
See all TNF Receptor II kits -
Assay type
ELISA set -
Range
15.625 pg/ml - 1000 pg/ml -
Product overview
The Antibody Pair can be used to quantify Human sTNF RII. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab260061), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human sTNF RII (TNFRSF1B) Capture Antibody (unconjugated) 1 x 100µg Human sTNF RII (TNFRSF1B) Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Post-translational
modificationsPhosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- CD120b
- p75
- p75 TNF receptor
see all
Images
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Sandwich ELISA - Human sTNF RII (TNFRSF1B) Antibody Pair - BSA and Azide free (ab267685)To learn more about the advantages of recombinant antibodies see here.
