Human SAP ELISA Kit (APCS) (ab137970)
Key features and details
- Sensitivity: 0.06 ng/ml
- Range: 0.156 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SAP ELISA Kit (APCS)
See all Serum Amyloid P/SAP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall = 4.4% Inter-assay Sample n Mean SD CV% Overall = 9.7% -
Sample type
Cell culture supernatant, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.06 ng/ml -
Range
0.156 ng/ml - 10 ng/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Serum 89% - 106% Plasma 99% - 105% -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human SAP (APCS) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of human SAP in plasma, serum, urine, and cell culture supernatants.
A SAP specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a SAP specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of SAP captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human SAP Antibody 1 x 120µl Chromogen Substrate 1 x 7ml SAP Microplate (12 x 8 well strips) 1 unit SAP Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. May also function as a calcium-dependent lectin. -
Tissue specificity
Found in serum and urine. -
Involvement in disease
Note=SAP is a precursor of amyloid component P which is found in basement membrane and associated with amyloid deposits. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Post-translational
modificationsN-glycosylated with a complex biantennary oligosaccharide chain with a sialic acid at the end (disialo-SAP). Monosialo-SAP as well as asioalo-SAP are also detected (PubMed:15174148). -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- 9.5S alpha 1 glycoprotein
- 9.5S alpha-1-glycoprotein
- Amyloid P component serum
see all -
Database links
- Entrez Gene: 325 Human
- Omim: 104770 Human
- SwissProt: P02743 Human
- Unigene: 507080 Human
Images
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Sandwich ELISA - ab137970 Serum Amyloid P (APCS) Human ELISA Kit
SAP measured in various samples showing quantity (microgram) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab137970.