Anti-ECE2 antibody (ab217350)
Key features and details
- Rabbit polyclonal to ECE2
- Suitable for: WB, IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-ECE2 antibody
See all ECE2 primary antibodies -
Description
Rabbit polyclonal to ECE2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseWB Mouse
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Immunogen
Synthetic peptide within Human ECE2 aa 780-830 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O60344 -
Positive control
- WB: Mouse brain lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ECE2 antibody (ab217350)PE Anti-CD4 antibody [RM4-5] (ab210350) at 1/300 dilution + mouse brain lysate
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 100 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ECE2 antibody (ab217350)Immunohistochemistry (Formalin/paraffin-embedded sections) analysis of mouse brain tissue labelling ECE2 with ab217350 at a dilution of 1/400. Antigen retrieval was performed by boiling in sodium citrate (pH6.0) for 15 minutes, followed by blocking of endogenous peroxidase by 3% hydrogen peroxide for 20 minutes. Normal goat serum was used as the blocking buffer at 37°C for 30 minutes followed by incubation with the primary antibody overnight at 4°C. DAB staining was performed.
