Human PRSS1 ELISA Kit (ab277474)
Key features and details
- Sensitivity: 0.29 ng/ml
- Range: 0.274 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PRSS1 ELISA Kit
See all PRSS1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.29 ng/ml -
Range
0.274 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 119.5 100% - 140% Plasma 120.3 99% - 146% Cell culture media 100.7 87% - 121% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human PRSS1 ELISA Kit (ab277474) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PRSS1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human PRSS1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PRSS1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PRSS1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PRSS1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent C 1 x 30ml Biotinylated Anti-Human PRSS1 Antibody 2 vials Anti-Human PRSS1 coated Microplate 1 unit Human PRSS1 standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Alpha-trypsin chain 2
- Beta-trypsin
- Cationic trypsinogen
see all -
Database links
- Entrez Gene: 5644 Human
- Omim: 276000 Human
- SwissProt: P07477 Human
- Unigene: 449281 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.