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Human MALT1 (MLT) knockout HeLa cell pellet (ab278901)

Overview

  • Product name

    Human MALT1 (MLT) knockout HeLa cell pellet
    See all MALT1/MLT kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.

  • Tissue specificity

    Highly expressed in peripheral blood mononuclear cells. Detected at lower levels in bone marrow, thymus and lymph node, and at very low levels in colon and lung.

  • Involvement in disease

    Note=A chromosomal aberration involving MALT1 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(11;18)(q21;q21) with BIRC2. This translocation is found in approximately 50% of cytogenetically abnormal low-grade MALT lymphoma.

  • Sequence similarities

    Belongs to the peptidase C14B family.
    Contains 1 death domain.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

  • Cellular localization

    Cytoplasm > perinuclear region. Nucleus. Shuttles between the nucleus and cytoplasm. Found in perinuclear structures together with BCL10.
  • Information by UniProt

  • Alternative names

    • Caspase like protein
    • DKFZp434L132
    • IMD12
    • MALT 1
    • MALT associated translocation
    • MALT lymphoma associated translocation
    • MALT lymphoma-associated translocation
    • Malt1
    • MALT1 paracaspase
    • MALT1_HUMAN
    • MLT
    • MLT 1
    • MLT1
    • Mucosa associated lymphoid tissue lymphoma translocation gene 1
    • Mucosa associated lymphoid tissue lymphoma translocation protein 1
    • Mucosa-associated lymphoid tissue lymphoma translocation protein 1
    • Paracaspase
    • Paracaspase-1
    • PCASP1
    see all

Properties

Images

  • Western blot - Human MALT1 (MLT) knockout HeLa cell pellet (ab278901)
    Western blot - Human MALT1 (MLT) knockout HeLa cell pellet (ab278901)

    Lane 1: Wild-type HeLa cell lysate (20 µg)

    Lane 2: MALT1 knockout HeLa cell lysate (20 µg)

    Lane 3: Ramos cell lysate (20 µg)

    Lanes 1-3: Merged signal (red and green). Green - ab33921 observed at 92 kDa. Red - loading control ab7291 observed at 50 kDa.

    ab33921 Anti-MALT1/MLT antibody [EP603Y] was shown to specifically react with MALT1/MLT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264930 (knockout cell lysate ab257149) was used. Wild-type and MALT1/MLT knockout samples were subjected to SDS-PAGE. ab33921 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human MALT1 (MLT) knockout HeLa cell pellet (ab278901)
    Sanger Sequencing - Human MALT1 (MLT) knockout HeLa cell pellet (ab278901)
    Homozygous: Insertion of the selection cassette in exon 1

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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