Human LKB1 ELISA Kit (ab233612)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 ng/ml
- Range: 3.13 ng/ml - 200 ng/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human LKB1 ELISA Kit
See all LKB1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% HEK293T 5 3.6% Inter-assay Sample n Mean SD CV% HEK293T 3 2.7% -
Sample type
Cell culture extracts, Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
3.13 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 100 94% - 108% Tissue 108 100% - 118% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human LKB1 ELISA Kit (ab233612) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LKB1 protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human LKB1 with 1 ng/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human LKB1 Capture Antibody 1 x 600µl 10X Human LKB1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human LKB1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor. -
Tissue specificity
Ubiquitously expressed. Strongest expression in testis and fetal liver. -
Involvement in disease
Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer.
Defects in STK11 have been associated with testicular tumors (TEST) [MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylated by a cAMP-dependent protein kinase. -
Cellular localization
Nucleus. Cytoplasm. Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2. - Information by UniProt
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Alternative names
- hLKB1
- Liver kinase B1
- LKB1
see all -
Database links
- Entrez Gene: 6794 Human
- Omim: 602216 Human
- SwissProt: Q15831 Human
- Unigene: 515005 Human
Images
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Other - Human LKB1 ELISA Kit (ab233612)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human LKB1 standard curve in 1X Cell Extraction Buffer PTR.The LKB1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native LKB1 in human MCF-7 cell extract based on a 3,000 µg/mL extract load and HEK293T cell extract based on a 1,500 µg/mL extract load.The concentrations of LKB1 were measured in duplicate and interpolated from the LKB1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LKB1 concentration was determined to be 27 ng/mL in MCF-7 cell extract and 46.3 ng/mL in HEK293T cell extract.
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Interpolated concentrations of native LKB1 in mouse RAW264.7 and rat PC-12 cell extract, extract based on a 750 µg/mL extract load.The concentrations of LKB1 were measured in duplicate and interpolated from the LKB1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LKB1 concentration was determined to be 61 ng/mL in mouse RAW 264.7 cell extract and 11 ng/mL in rat PC12.
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Sandwich ELISA - Human LKB1 ELISA Kit (ab233612)To learn more about the advantages of recombinant antibodies see here.
