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Human KDM6B (JMJD3) knockout HEK293T cell pellet (ab279133)

Overview

  • Product name

    Human KDM6B (JMJD3) knockout HEK293T cell pellet
    See all KDM6B / JMJD3 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 5.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

  • Sequence similarities

    Belongs to the UTX family.
    Contains 1 JmjC domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Alternative names

    • Histone demethylase JMJD3
    • JmjC domain containing protein 3
    • JmjC domain-containing protein 3
    • Jumonji D3
    • Jumonji domain containing 3
    • Jumonji domain containing 3 histone lysine demethylase
    • Jumonji domain containing protein 3
    • Jumonji domain-containing protein 3
    • Kdm6b
    • KDM6B_HUMAN
    • KIAA0346
    • Lysine demethylase 6B
    • Lysine K specific demethylase 6B
    • Lysine specific demethylase 6B
    • Lysine-specific demethylase 6B
    see all

Properties

Images

  • Sanger Sequencing - Human KDM6B (JMJD3) knockout HEK293T cell pellet (ab279133)
    Sanger Sequencing - Human KDM6B (JMJD3) knockout HEK293T cell pellet (ab279133)
    Homozygous: 14 bp deletion in exon 5

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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