Human Interferon alpha 1 ELISA Kit (ab213479)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 4.68 pg/ml - 300 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Interferon alpha 1 ELISA Kit
See all Interferon alpha 1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 2% Inter-assay Sample n Mean SD CV% Overall 5 3% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
4.68 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 87% - 92% Cell culture extracts 105 102% - 108% Cell culture media 93 92% - 94% Hep Plasma 91 85% - 98% EDTA Plasma 87 81% - 90% Cit plasma 77 76% - 78% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Interferon alpha 1 ELISA Kit (ab213479) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon alpha 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon alpha 1 with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interferon alpha-1 belongs to a group of functionally related but distinct proteins of interferon alpha family that share around 80% identity of their amino acid sequences. Members of the interferon-alpha family have both anti-viral and immunomodulatory activities on target cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Interferon alpha-1 Capture Antibody 1 x 600µl 10X Human Interferon alpha-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Interferon alpha-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IFL
- IFN
- IFN alpha
see all -
Database links
- Entrez Gene: 3439 Human
- Entrez Gene: 3447 Human
- Omim: 147660 Human
- SwissProt: P01562 Human
- Unigene: 37026 Human
- Unigene: 533471 Human
Images
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Other - Human Interferon alpha 1 ELISA Kit (ab213479)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Interferon alpha-1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Interferon alpha-1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.
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Interpolated concentrations of native Interferon alpha-1 in WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784.
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Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD, n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.
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Sandwich ELISA - Human Interferon alpha 1 ELISA Kit (ab213479)To learn more about the advantages of recombinant antibodies see here.
