Anti-IRF3 antibody (ab25950)
Key features and details
- Rabbit polyclonal to IRF3
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-IRF3 antibody
See all IRF3 primary antibodies -
Description
Rabbit polyclonal to IRF3 -
Host species
Rabbit -
Specificity
ab25950 recognises IRF3. -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
A synthetic peptide corresponding to 14 amino acids near the carboxyterminus of IRF3 (Human)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
IRF3 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRF3 antibody (ab25950)Lane 1 : Anti-IRF3 antibody (ab25950) at 1 µg/ml
Lane 2 : Anti-IRF3 antibody (ab25950) at 2 µg/ml
Lane 3 : Anti-IRF3 antibody (ab25950) at 4 µg/ml
All lanes : Ramos whole cell lysate.
Predicted band size: 47 kDa
Observed band size: ~44 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody (ab25950)ab25950 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab25950 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-IRF3 antibody (ab25950) This image is courtesy of an anonymous AbreviewAnti-IRF3 antibody (ab25950) at 1/1000 dilution + HT-29 at 30 µg
Secondary
HRP conjugated Anti-rabbit IgG, Goat polyclonal at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDaSamples were incubated with primary antibody for 18 hours at 4ºC and blocked with 5% BSA for 1 hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-IRF3 antibody (ab25950)Immunofluorescence of IRF3 in Mouse Kidney cells using ab25950 at 20 ug/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF3 antibody (ab25950)ab25950 at 2µg/ml staining IRF3 in mouse kidney by IHC.
