Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 1.8 pg/ml - 1200 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human Interferon alpha 1 ELISA Kit, Fluorescent
See all Interferon alpha 1 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% HEK293T 3 2% Inter-assay Sample n Mean SD CV% HEK293T 5 3% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
1.8 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 93 92% - 94% Serum 89 87% - 92% Cell culture extracts 105 102% - 108% Hep Plasma 91 85% - 98% EDTA Plasma 87 81% - 90% Cit plasma 77 76% - 78% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Interferon alpha-1 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon alpha-1 protein in human serum, plasma, cell culture supernatants, and cell and tissue extracts.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
-
Notes
Interferon alpha-1 belongs to a group of functionally related but distinct proteins of interferon alpha family that share around 80% identity of their amino acid sequences. Members of the interferon-alpha family have both anti-viral and immunomodulatory activities on target cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human Interferon alpha-1 Capture Antibody 1 x 600µl 10X Human Interferon alpha-1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human Interferon alpha-1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
-
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- IFL
- IFN
- IFN alpha
see all -
Database links
- Entrez Gene: 3439 Human
- Entrez Gene: 3447 Human
- Omim: 147660 Human
- SwissProt: P01562 Human
- Unigene: 37026 Human
- Unigene: 533471 Human
Images
-
Other - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human Interferon alpha-1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of spike Interferon alpha-1 in human plasma samples.The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 100%, plasma (citrate) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Interpolated concentrations of spike Interferon alpha-1 in human serum and cell culture media samples.The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, cell culture media (RPMI1640 + 10% FBS) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.
-
Interpolated concentrations of native Interferon alpha-1 in WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784.
-
Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD, n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.
-
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)To learn more about the advantages of recombinant antibodies see here.
