Human IL-22RA2 ELISA kit (IL-22BP) (ab267601)
Key features and details
- Sensitivity: 1.64 ng/ml
- Range: 1.638 ng/ml - 400 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-22RA2 ELISA kit (IL-22BP) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.64 ng/ml -
Range
1.638 ng/ml - 400 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 129.3 109% - 148% Plasma 126.9 108% - 146% Cell culture media 130 119% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-22RA2 ELISA Kit is designed for the quantitative determination of IL-22RA2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human IL-22RA2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-22RA2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-22RA2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-22RA2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain, Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-23R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Semaphorin 7A, Siglec-11.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human IL-22RA2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human IL-22RA2 Detection Antibody 2 vials Human IL-22RA2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
Isoform 1 may play a role in establishing and maintaining successful pregnancy. -
Tissue specificity
Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placenta. Isoform 2 is expressed in placenta and breast and at lower level in spleen, skin, thymus and stomach. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- IL 22BP
- IL 22RA2
- IL22RA2
see all -
Database links
- Entrez Gene: 116379 Human
- Omim: 606648 Human
- SwissProt: Q969J5 Human
- Unigene: 126891 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.