Human Granzyme B ELISA Kit (CTLA-1) (ab46142)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 31.25 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 15ml 1 x 25ml 200X Wash Buffer White 1 x 10ml 2 x 10ml Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml Biotinylated anti-Granzyme B Red 1 x 400µl 2 x 400µl Chromogen TMB Substrate Solution 1 x 11ml 1 x 24ml Granzyme B Microplate (12 x 8 well strips) 1 unit 2 units Granzyme B Standard (Lyophilized) Yellow 2 vials 4 vials HRP Diluent Red 1 x 12ml 1 x 23ml Stop Reagent Black 1 x 11ml 2 x 11ml Streptavidin-HRP 2 x 5µl 4 x 5µl -
Research areas
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Relevance
The lytic enzymes of cytotoxic lymphocytes seem to be contained within lysosome-like granules found in the cytoplasm. These granules contain perforin, a membrane pore-forming protein, and a family of serine proteases (granzymes). Granzyme B, a 32 kDa protein, is a serine Aspase associated with the granules of Natural Killer (NK) cells. Granzyme B seems to be able to elicit cell death, perhaps by activating an endogenous pathway of programmed cell death. -
Cellular localization
Cytoplasmic -
Alternative names
- C11
- CTLA 1
- Fragmentin 2
see all -
Database links
- Entrez Gene: 3002 Human
- SwissProt: P10144 Human
Images
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Sandwich ELISA - Hu Granzyme B (ab46142)
Hu Granzyme B (CTLA-1) measured in biologicals showing quantity (pg) per mL of tested sample
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Sandwich ELISA - Hu Granzyme B (ab46142)Representative Standard Curve using ab46142
