Human Glycogen Synthase 1 Matched Antibody Pair Kit (ab219529)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human Glycogen Synthase 1 Matched Antibody Pair Kit
See all Glycogen synthase 1/GYS1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
10.7 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Glycogen Synthase 1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Glycogen Synthase 1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab214031. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Glycogen Synthase 1 Capture Antibody 2 x 50µg 1 x 50µg Human Glycogen Synthase 1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Glycogen Synthase 1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. -
Pathway
Glycan biosynthesis; glycogen biosynthesis. -
Involvement in disease
Defects in GYS1 are the cause of muscle glycogen storage disease type 0 (GSD0b) [MIM:611556]; also known as muscle glycogen synthase deficiency. GSD0b is a metabolic disorder characterized by fasting hypoglycemia presenting in infancy or early childhood. The role of muscle glycogen is to provide critical energy during bursts of activity and sustained muscle work. -
Sequence similarities
Belongs to the glycosyltransferase 3 family. - Information by UniProt
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Alternative names
- Glycogen [starch] synthase
- Glycogen synthase 1
- Glycogen synthase 1 (muscle)
see all -
Database links
- Entrez Gene: 2997 Human
- Omim: 138570 Human
- SwissProt: P13807 Human
- Unigene: 386225 Human
Images
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Human Glycogen Synthase 1 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Glycogen Synthase 1 Matched Antibody Pair Kit (ab219529)To learn more about the advantages of recombinant antibodies see here.
