Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human Glycogen Synthase 1 ELISA Kit (ab214031)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 14 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture extracts, Plasma, Serum, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Glycogen Synthase 1 ELISA Kit
    See all Glycogen synthase 1/GYS1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    HeLa extract 5 2.5%
    Inter-assay
    Sample n Mean SD CV%
    HeLa extract 3 5.6%

  • Sample type

    Serum, Plasma, Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    14 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 80 76% - 84%
    Tissue Extracts 108 105% - 112%
    Hep Plasma 81 77% - 83%
    EDTA Plasma 95 90% - 97%
    Cit plasma 89 85% - 92%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Glycogen Synthase 1 ELISA Kit (ab214031) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Glycogen Synthase 1 protein in tissue extracts, plasma, serum, and cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Glycogen Synthase 1 with 14 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Glycogen Synthase 1 (also known as Glycogen [starch] synthase, muscle) is a glycosyltransferase enzyme involved in converting glucose to glycogen. Mutations in Glycogen Synthase 1 are associated with glycogen storage disease. Mouse and rat Glycogen Synthase 1 have 97% protein sequence identity to human Glycogen Synthase 1.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Glycogen Synthase 1 ELISA Kit (ab214031)
    Other - Human Glycogen Synthase 1 ELISA Kit (ab214031)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of Glycogen Synthase 1 standard curve in Sample Diluent NS.
    Example of Glycogen Synthase 1 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of Glycogen Synthase 1 standard curve in 1X Cell Extraction Buffer PTR.
    Example of Glycogen Synthase 1 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Glycogen Synthase 1 in Human HELA, HEK293, and Liver Extract.
    Interpolated concentrations of native Glycogen Synthase 1 in Human HELA, HEK293, and Liver Extract.

    The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 38 pg/µg HELA extract, 129 pg/µg HEK293 extract and 5.9 pg/µg Liver extract.

  • Interpolated concentrations of native Glycogen Synthase 1 in mouse NIH3T3 extract and rat PC12 extract.
    Interpolated concentrations of native Glycogen Synthase 1 in mouse NIH3T3 extract and rat PC12 extract.

    The concentrations of Glycogen Synthase 1 were measured in duplicate and interpolated from the Glycogen Synthase 1 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Glycogen Synthase 1 concentration was determined to be 5.7 pg/µg NIH3T3 extract and 595 pg/µg PC12 extract

  • Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (ab214031)
    Sandwich ELISA - Human Glycogen Synthase 1 ELISA Kit (ab214031)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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