Human FGFR-4 ELISA Kit (ab256399)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 15 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture media, Cell culture supernatant, Cell Lysate, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Macaque monkey
Overview
-
Product name
Human FGFR-4 ELISA Kit
See all FGFR4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.7% Inter-assay Sample n Mean SD CV% Serum 3 2.8% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Cell culture media, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
15 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 109 104% - 115% Serum 116 115% - 119% Cell Lysate 151 143% - 156% Cell culture media 86 83% - 93% EDTA Plasma 114 109% - 121% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Macaque monkey
Does not react with: Cow -
Product overview
Human FGFR-4 ELISA Kit (ab256399) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGFR-4 protein in cell culture supernatant, cell lysate, edta plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FGFR-4 with 15 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
FGFR-4 (Fibroblast growth factor receptor 4) is a tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. FGFR-4 mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling. FGFR-4 is highly homologous among the species, rhesus macaque FGFR-4 is 97% identical on amino acid level.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human FGFR-4 Capture Antibody 1 x 600µl 10X Human FGFR-4 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human FGFR-4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19. -
Tissue specificity
Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsGlycosylated.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization. -
Cellular localization
Membrane. Isoform 2 may be secreted. - Information by UniProt
-
Alternative names
- CD 334
- CD334
- CD334 antigen
see all -
Database links
- Entrez Gene: 2264 Human
- Omim: 134935 Human
- SwissProt: P22455 Human
- Unigene: 165950 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human FGFR-4 standard curve in Sample Diluent NS.The FGFR-4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native FGFR-4 in human serum, plasma and cell culture supernatant samples.The concentrations of FGFR-4 were measured in duplicates, interpolated from the FGFR-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 12.5% and HepG2 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 11,887 pg/mL in neat serum, 27,375 pg/mL in neat plasma (EDTA) and 11,607 pg/mL in neat HepG2 cell culture supernatant.
-
Interpolated concentrations of native FGFR-4 in 250 µg/mL HepG2 cell extract.The concentrations of FGFR-4 were measured in duplicate and interpolated from the FGFR-4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 6,588 pg/mL in 250 µg/mL HepG2 cell extract.
-
Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGFR-4 concentration was determined to be 8,757 pg/mL with a range of 3,634 – 33,733 pg/mL.
-
Sandwich ELISA - Human FGFR-4 ELISA Kit (ab256399)To learn more about the advantages of recombinant antibodies see here.
