Recombinant human Visfatin protein (ab198090)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human Visfatin protein
See all Visfatin proteins and peptides -
Biological activity
Specific Activity: 2 pmol/min/µg.
Assay conditions: The 50 µl reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 µg/ml of alcohol dehydrogenase, 10 µg/mL of NMNAT, 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 min, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured. -
Purity
>= 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
GPLGSNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTEN SKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVF NEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTN WIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYR GVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAE HSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLR HLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPP YLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLN CSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEG KGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH -
Predicted molecular weight
56 kDa -
Amino acids
2 to 491 -
Additional sequence information
NM_005746.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human Visfatin protein (ab198090)
Activity assay using ab198090.
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SDS-PAGE - Recombinant human Visfatin protein (ab198090)4-20% SDS-PAGE analysis of 2 µg of ab198090 with Coomassie staining.
