Rat Clusterin ELISA Kit (ab231934)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.63 µg/ml
- Range: 0.8 ng/ml - 50 ng/ml
- Sample type: Cit plasma, Hep Plasma, Other biological fluids, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat Clusterin ELISA Kit
See all Clusterin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rat plasma 5 5.5% Inter-assay Sample n Mean SD CV% Rat plasma 3 4.2% -
Sample type
Serum, Other biological fluids, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.63 µg/ml -
Range
0.8 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 96% - 108% Other biological fluids 108 101% - 117% Hep Plasma 103 99% - 107% Cit plasma 110 103% - 123% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat Clusterin ELISA Kit (ab231934) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Clusterin protein in hep plasma, other biological fluids, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Clusterin with 0.63 µg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat Clusterin Capture Antibody 1 x 600µl 10X Rat Clusterin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat Clusterin Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. -
Tissue specificity
Detected in blood plasma, cerebrospinal fluid, milk, seminal plasma and colon mucosa. Detected in the germinal center of colon lymphoid nodules and in colon parasympathetic ganglia of the Auerbach plexus (at protein level). Ubiquitous. Detected in brain, testis, ovary, liver and pancreas, and at lower levels in kidney, heart, spleen and lung. -
Sequence similarities
Belongs to the clusterin family. -
Post-translational
modificationsIsoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen.
Polyubiquitinated, leading to proteasomal degradation.
Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate. -
Cellular localization
Secreted. Can retrotranslocate from the secretory compartments to the cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytoplasmic vesicle, secretory vesicle, chromaffin granule. Isoforms lacking the N-terminal signal sequence have been shown to be cytoplasmic and/or nuclear. Secreted isoforms can retrotranslocate from the secretory compartments to the cytosol upon cellular stress. Detected in perinuclear foci that may be aggresomes containing misfolded, ubiquitinated proteins. Detected at the mitochondrion membrane upon induction of apoptosis. - Information by UniProt
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Alternative names
- 40
- AAG 4
- AAG4
see all -
Database links
- Entrez Gene: 24854 Rat
- SwissProt: P05371 Rat
- Unigene: 1780 Rat
Images
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Other - Rat Clusterin ELISA Kit (ab231934)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat Clusterin standard curve in Sample Diluent NS.The Clusterin standard curve was prepared as described in Section 10.
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Interpolated concentrations of native Clusterin in rat serum and plasma samples.The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 4,000, plasma (citrate) 1: 4,000, and plasma (heparin) 1: 4,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 87 µg/mL in serum, 102 µg/mL in plasma (citrate), and 119 µg/mL in plasma (heparin).
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Interpolated concentrations of native Clusterin in rat tissue supernatant samples.The concentrations of Clusterin were measured in duplicates, interpolated from the Clusterin standard curves and corrected for sample dilution. Undiluted samples are as follows: liver supernatant 40% and stomach supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 102 ng/ml in liver supernatant and 62 ng/mL in stomach supernatant.
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Serum from five individual healthy rat female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Clusterin concentration was determined to be 172 µg/mL with a range of 155 – 189 µg/mL.
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Sandwich ELISA - Rat Clusterin ELISA Kit (ab231934)To learn more about the advantages of recombinant antibodies see here.
