Human Elafin ELISA Kit (Skalp) (ab100658)
Key features and details
- Sensitivity: 5 pg/ml
- Range: 6.86 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Elafin ELISA Kit (Skalp)
See all Elafin/ESI kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 5 pg/ml -
Range
6.86 pg/ml - 5000 pg/ml -
Recovery
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 111.8 102% - 121% Serum 115.7 105% - 125% Plasma 112.5 103% - 122% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Elafin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Elafin in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human Elafin coated on a 96-well plate. Standards and samples are pipetted into the wells and Elafin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Elafin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Elafin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Biotinylated anti-Human Elafin (lyophilized) 2 vials Elafin Microplate (12 x 8 wells) 1 unit Recombinant Human Elafin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. -
Sequence similarities
Contains 1 WAP domain. -
Domain
Consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Cementoin
- ELAF_HUMAN
- Elafin
see all -
Database links
- Entrez Gene: 5266 Human
- Omim: 182257 Human
- SwissProt: P19957 Human
- Unigene: 112341 Human
Images
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Typical standard curve
Representative standard curve using ab100658
