Human CDK1 Matched Antibody Pair Kit (ab219530)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 125 pg/ml - 8000 pg/ml
Overview
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Product name
Human CDK1 Matched Antibody Pair Kit
See all CDK1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
24.17 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human CDK1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human CDK1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab212162. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human CDK1 Capture Antibody 2 x 50µg 1 x 50µg Human CDK1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human CDK1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. -
Tissue specificity
Isoform 2 is found in breast cancer tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Cdc 2
- Cdc2
- CDC28A
see all -
Database links
- Entrez Gene: 983 Human
- Omim: 116940 Human
- SwissProt: P06493 Human
- Unigene: 334562 Human
Images
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Human CDK1 standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human CDK1 Matched Antibody Pair Kit (ab219530)To learn more about the advantages of recombinant antibodies see here.
