Human CD200R ELISA Kit (CD200R1) (ab272196)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.89 pg/ml
- Range: 46.88 pg/ml - 3000 pg/ml
- Sample type: Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human CD200R ELISA Kit (CD200R1)
See all CD200R kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 7.5% Inter-assay Sample n Mean SD CV% Serum 3 7.7% -
Sample type
Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.89 pg/ml -
Range
46.88 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 115 112% - 120% Cell Lysate 99 90% - 116% Hep Plasma 91 81% - 99% EDTA Plasma 93 81% - 110% Cit plasma 92 83% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD200R ELISA kit (ab272196) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CD200R protein in serum, plasma and cell and tissue culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CD200R with 11.89 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
CD200R (CD200R1), is a transmembrane glycoprotein member of the immunoglobin superfamily. CD200R acts as an inhibitory receptor for the CD200/OX-2 cell surface glycoprotein. CD200R is mainly expressed in myeloid cells and CD4+ T-cells and known to interact with CD200 in variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells. Activation of CD200R regulates the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase. Recent studies suggest that CD200R may prove useful in monitoring changes in bone metabolism. Mouse, rat and cow share 57%, 57% and 60% sequence homology with human CD200R.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CD200R (CD200R1) Capture Antibody 1 x 600µl 10X Human CD200R (CD200R1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CD200R (CD200R1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. -
Tissue specificity
Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. -
Sequence similarities
Belongs to the CD200R family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsThe mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
-
Alternative names
- CD 200R
- CD200 cell surface glycoprotein receptor
- CD200 receptor 1
see all -
Database links
- Entrez Gene: 131450 Human
- Omim: 607546 Human
- SwissProt: Q8TD46 Human
- Unigene: 309158 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human CD200R standard curve in Sample Diluent NS.The CD200R standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native CD200R in human serum, and plasma samples.The concentrations of CD200R were measured in duplicates, interpolated from the CD200R standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 2,595 pg/mL in serum, 2,460 pg/mL in plasma (citrate), 2,904 pg/mL in plasma (EDTA), and 3,137 pg/mL in plasma (heparin).
-
Interpolated concentrations of native CD200R in human HEL cell extract based on a 500 µg/mL extract load.The concentrations of CD200R were measured in duplicate and interpolated from the CD200R standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CD200R concentration was determined to be 817.1 pg/mL in HEL cell extract sample.
-
Sandwich ELISA - Human CD200R ELISA Kit (CD200R1) (ab272196)To learn more about the advantages of recombinant antibodies see here.
