Human CD157 ELISA kit (ab267623)
Key features and details
- Sensitivity: 0.06 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD157 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.06 ng/ml -
Range
0.051 ng/ml - 12.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 130.9 124% - 138% Plasma 129.8 113% - 145% Cell culture media 123.4 112% - 140% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD157 ELISA Kit is designed for the quantitative determination of CD157 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human CD157 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD157 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD157 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD157 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human AMIGO, Aminopeptidase LRAP (ERAP2), Amnionless, Arylsulfatase A, Bcl-w, CD109, CD34, CD83, CLEC-1, CLEC10A (CD301), CMG-2, CREG, Cystatin SN, Cytokeratin-8, Dectin-1 (CLEC7A), Desmocollin-3, Endoglycan, Galectin-4, HAPLN1, Jagged 1, Langerin (CD207), Lumican, Matriptase (ST14), MEP1B, Nectin-3, OX40 (TNFRSF4), OX40 Ligand (TNFSF4), p27, Pappalysin-2, Plexin B3, Plexin D1, proGRP, PSA-total, Reg1B, RGM-A, ROBO2, Spinesin, TWEAK R (TNFRSF12), ULBP-3.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human CD157 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human CD157 Detection Antibody 2 vials Human CD157 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Synthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger that elicits calcium release from intracellular stores. May be involved in pre-B-cell growth. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the ADP-ribosyl cyclase family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- ADP ribosyl cyclase 2
- ADP-ribosyl cyclase 2
- ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2
see all -
Database links
- Entrez Gene: 683 Human
- Omim: 600387 Human
- SwissProt: Q10588 Human
- Unigene: 720344 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.