Human BHMT ELISA Kit (ab221830)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 9.34 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BHMT ELISA Kit
See all BHMT kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 3.1% Inter-assay Sample n Mean SD CV% Overall 5 8.3% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
9.34 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 107 105% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human BHMT ELISA Kit (ab221830) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BHMT protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BHMT with 9.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
BHMT is a zinc metalloenzyme that belongs to the methyltransferase family. Its function is to catalyze the transfer of a methyl group from trimethylglycine and a hydrogen ion from homocysteine to produce dimethylglycine and methionine. It is involved in the metabolism of glycine, serine, threonine and methionine.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BHMT Capture Antibody 1 x 600µl 10X Human BHMT Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human BHMT Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. -
Tissue specificity
Found exclusively in liver and kidney. -
Pathway
Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1. -
Sequence similarities
Contains 1 Hcy-binding domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Betaine homocysteine methyltransferase
- Betaine homocysteine S methyltransferase
- Betaine homocysteine S methyltransferase 1
see all -
Database links
- Entrez Gene: 635 Human
- Omim: 602888 Human
- SwissProt: Q93088 Human
- Unigene: 80756 Human
Images
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Other - Human BHMT ELISA Kit (ab221830)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human BHMT standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native BHMT in human, rat and mouse samples based on a 31, 6.3, and 0.5 µg/mL extract load.The concentrations of BHMT standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BHMT concentration was determined to be 1373 pg/mL in human liver extracts, 1282 pg/mL in rat liver extracts and 985 pg/mL in mouse liver extracts.
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Sandwich ELISA - Human BHMT ELISA Kit (ab221830)To learn more about the advantages of recombinant antibodies see here.
