Anti-IDH3B antibody (ab247089)
Key features and details
- Rabbit polyclonal to IDH3B
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IDH3B antibody -
Description
Rabbit polyclonal to IDH3B -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Cynomolgus monkey, Orangutan
-
Immunogen
Recombinant fragment corresponding to Human IDH3B aa 50-200.
Database link: O43837 -
Positive control
- IHC-P: Human parathyroid gland, pancreas, kidney, placenta and testis tissue. WB: RT4 and U-251 MG cell lysates; Human tonsil lysate. ICC/IF: A549 cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-IDH3B antibody (ab247089)
PFA-fixed, Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells stained for IDH3B (green) using ab247089 at 4 µg/ml in ICC/IF.
-
Western blot - Anti-IDH3B antibody (ab247089)All lanes : Anti-IDH3B antibody (ab247089) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human rain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 42 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)Formalin-fixed, paraffin-embedded human testis tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)Formalin-fixed, paraffin-embedded human kidney tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)Formalin-fixed, paraffin-embedded human pancreas tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)Formalin-fixed, paraffin-embedded human placenta tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IDH3B antibody (ab247089)Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for IDH3B with ab247089 at a 1/2500 dilution in immunohistochemical analysis.