Human BHMT Matched Antibody Pair Kit (ab222261)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human BHMT Matched Antibody Pair Kit
See all BHMT kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
9.1 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human BHMT Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human BHMT.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab221830. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human BHMT Capture Antibody 2 x 50µg 1 x 50µg Human BHMT Detector Antibody 2 x 12.5µg 1 x 12.5µg Human BHMT Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. -
Tissue specificity
Found exclusively in liver and kidney. -
Pathway
Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1. -
Sequence similarities
Contains 1 Hcy-binding domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Betaine homocysteine methyltransferase
- Betaine homocysteine S methyltransferase
- Betaine homocysteine S methyltransferase 1
see all -
Database links
- Entrez Gene: 635 Human
- Omim: 602888 Human
- SwissProt: Q93088 Human
- Unigene: 80756 Human
Images
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Human BHMT Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human BHMT Matched Antibody Pair Kit (ab222261)To learn more about the advantages of recombinant antibodies see here.
