HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)
![HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)](https://www.abcam.com/ps/products/9/ab9482/Images/ab9482-343848-ab9482DS3.jpg "HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)")
Key features and details
- HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control
See all GAPDH primary antibodies -
Description
HRP Mouse monoclonal [mAbcam 9484] to GAPDH - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length native protein (purified) corresponding to Human GAPDH.
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Positive control
- WB: HeLa, HEK-293, NIH/3T3 and PC-12 whole cell lysates.
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General notes
According to our customer's feedback this antibody does not recognise meningococcal GapA1 or GapA2 (GAPDH) recombinant proteins.
This antibody has been conjugated with HRP. The concentration of the original antibody was 1 mg/ml, following conjugation the concentration observed will be higher.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam 9484 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482)All lanes : HRP Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9482) at 1/1000 dilution
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Hek293 Whole Cell Lysate
Lane 3 : NIH 3T3 Whole Cel lLysate
Lane 4 : PC12 Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40.2 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
