HRP Anti-alpha 1 Antitrypsin antibody (ab191350)
Key features and details
- HRP Goat polyclonal to alpha 1 Antitrypsin
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-alpha 1 Antitrypsin antibody
See all alpha 1 Antitrypsin primary antibodies -
Description
HRP Goat polyclonal to alpha 1 Antitrypsin -
Host species
Goat -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Positive control
- WB: Human plasma. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against buffer. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-alpha 1 Antitrypsin antibody (ab191350)HRP Anti-alpha 1 Antitrypsin antibody (ab191350) at 1/5000 dilution + Human Plasma Total Protein at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsAlpha I Antitrypsin contains three potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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Immunocytochemistry/ Immunofluorescence - HRP Anti-alpha 1 Antitrypsin antibody (ab191350)ICC/IF image of ab191350 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab191350, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96931, DyLight® 488 donkey anti-goat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
